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C4899
Sigma
Carnitine Acetyltransferase from pigeon breast muscle
ammonium sulfate suspension, ≥70 units/mg protein
| Synonym: | Acetyl-Co-A:carnitine-O-acetyltransferase, CrAT |
| CAS Number: | 9029-90-7 |
| Enzyme Commission (EC) Number: | 2.3.1.7 ( BRENDA |
| MDL number: | MFCD00130725 |
Description
| Analysis Note | Protein determined by biuret. |
| Biochem/physiol Actions | Carnitine acetyltransferase maintains the cellular and mitochondrial levels of acetyl-CoA, a key cofactor required for oxidative metabolism, by catalyzing an equilibrium between acetyl-CoA and acetyl-L-carnitine, a storage form of activated acetate. Carnitine acetyltransferase also maintains the pool of acetyl-CoA required for neuronal and nonneuronal acetylcholine production. |
| Unit Definition | One unit will convert 1.0 μmole of acetyl- |
| Physical form | Crystalline suspension in 3.2 M (NH4)2SO4 solution, 50 mM potassium phosphate, 1 mM dithiothreitol, pH 7.0 |
Properties
| form | ammonium sulfate suspension |
| storage temp. | 2-8°C |
Safety
References
| reference | Ramsay, R.R., and Naismith, J.H., A snapshot of carnitine acetyltransferase. Trends Biochem. Sci. 28, 343-346, (2003) |
| Horiuchi, Y., et al., Evolutional study on acetylcholine expression. Life Sci. 72, 1745-1756, (2003) | |
| Bakker, A., et al., Ultrastructural localisation of carnitine acetyltransferase activity in mitochondria of rat myocardium. Biochim. Biophys. Acta 1185, 97-102, (1994) |



